Data Sheet 2

With ABIRD running, you can begin to further optimise the whole system to take advantage of the consistent low background ion signal.

This can be shown in a LOW LEVEL COMPLEX sample analysis, like a dilute fraction from a whole cell lysate digestion for instance. With ABIRD on, more PSMs were found, and at a lower signal level (blue bars) than with ABIRD off (red bars). This is 14 runs from a single vial with ABIRD alternating between on and off.

Can you do this? Probably not at first, many lab dependent factors are involved. This is actually a subtle difference between data sets, only 10% more PSMs with ABIRD. But see the intensity difference of the PSMs, and correlate how that would improve an even more complex low level data set.

ABIRD ON and OFF on a Thermo Orbitrap Velos Pro
ABIRD ON and OFF on a Thermo Orbitrap Velos Pro

Click for full size chart